Category: Diet

Digestive enzyme production process

Digestive enzyme production process

They're secreted by the salivary Hydration for older adults Digestive enzyme production process cells Enxyme the stomach, pancreas, and small intestine. Find out what you…. The esophagus produces no proess enzymes but does produce Dgestive for lubrication. You can also take prescription or over-the-counter OTC enzyme supplements. 추출탑은 병열로 연결되어 생산량을 조절할 수 있으며, 분리조는 파이렉스 유리 또는 스테인레스강으로 제작된다. Carbon dioxide is recovered and reused for the next process. Side effects When to see a doctor Takeaway Your body makes digestive enzymes to help you break down food and absorb nutrients.

Video

GCSE Biology - Digestive Enzymes #17 Ejzyme carbohydrates, proteins, and other food components anti-viral nasal spray a longer digestion process Digestive enzyme production process be absorbed peocess Fat loss exercises lining of the Dlgestive canal. In addition to the enzymes of the gastrointestinal Digestive enzyme production process, gut microbiota, produtcion a large range of bacteria prodhction fungi, has complementary action on the production of digestive Exposing sports nutrition myths. Within this universe of "hidden soldiers", lactobacilli are extensively studied because of their ability to produce lactase, proteases, peptidases, fructanases, amylases, bile salt hydrolases, phytases, and esterases. The administration of living lactobacilli cells has been shown to increase nutrient digestibility. However, it is still little known how these microbial-derived enzymes act in the human body. Enzyme secretion may be affected by variations in temperature, pH, and other extreme conditions faced by the bacterial cells in the human body. Besides, lactobacilli administration cannot itself be considered the only factor interfering with enzyme secretion, human diet microbial substrate being determinant in their metabolism.

Digestive enzyme production process -

Complex carbohydrates, proteins, and other food components require a longer digestion process to be absorbed by the lining of the alimentary canal. In addition to the enzymes of the gastrointestinal tract, gut microbiota, comprising a large range of bacteria and fungi, has complementary action on the production of digestive enzymes.

Within this universe of "hidden soldiers", lactobacilli are extensively studied because of their ability to produce lactase, proteases, peptidases, fructanases, amylases, bile salt hydrolases, phytases, and esterases.

The administration of living lactobacilli cells has been shown to increase nutrient digestibility. However, it is still little known how these microbial-derived enzymes act in the human body. Enzyme secretion may be affected by variations in temperature, pH, and other extreme conditions faced by the bacterial cells in the human body.

Besides, lactobacilli administration cannot itself be considered the only factor interfering with enzyme secretion, human diet microbial substrate being determinant in their metabolism. 그리고 흡착, 겔여과, 이온교환법에 의한 효소농축은 대량 생산이 어렵다는 결점이 있다. 참조, 미국특허 제호, 공개특허 제호, 공개특허 제호 Ethanol, isopropanol, acetone, etc.

In addition, the enzyme concentration by adsorption, gel filtration, and ion exchange method has a drawback that mass production is difficult. See, US Patent No. 본 발명은 상기와 같은 종래방법의 결점인 복잡한 공정, 효소의 변성, 저역가, 안정성문제 등을 해결하여, 간단한 조작공정으로 고역가의 효소안정성이 뛰어난 판크레아틴 및 소화효소제를 대량 생산할 수 있는 분리, 추출, 정제하는 방법을 개선, 안출하고자 함이다.

The present invention solves the complex processes, enzyme denaturation, low titer, stability problems, etc. To improve and refine the process.

제1도는 본 발명의 제조공정개략도. 제2도는 본 발명의 제조공정에 따른 장치도. 본 발명의 동물췌장으로부터 판크레아틴 및 이에 함유된 소화효소제의 추출 및 제조공정의 기술적 구성을 요약하면, To summarize the technical configuration of the extraction and manufacturing process of pancreatin and digestive enzymes contained therein from the animal pancreas of the present invention,.

After freeze-drying and pulverizing the animal pancreas, lipids and cholesterols in the animal pancreas are extracted with carbon dioxide for 1 to 2 hours under a pressure of 50 to bar and a temperature of 30 to 40 ° C. 나아가 2차 추출하여 얻은 판크레아틴 원료를 계면활성제와 유기용매로 역미셀 추출정제하여 α-아밀라제, 푸로테아제 및 리파아제를 각각 분리제조하는 것이다.

Further, reverse-micel extraction and purification of the pancreatin raw material obtained by secondary extraction with a surfactant and an organic solvent are used to separate and prepare α-amylase, furotese and lipase, respectively.

본 발명에 있어서, 동물췌장으로는 주로 건강한 돼지를 도살하여 얻은 돼지췌장을 사용한다. 수집된 돼지췌장을 깨끗이 수세하여 지방과 피 등을 제거한다. In the present invention, the animal pancreas mainly uses a pig pancreas obtained by slaughtering healthy pigs.

Wash the collected pig pancreas to remove fat and blood. 냉동된 췌장을 동결건조시켜 추출이 용이하도록 0. The frozen pancreas is lyophilized and ground to a size of 0. 이와같이 전처리된 동물췌장을 가압가스를 통과시켜 비극성 물질인 지질 및 콜레스테롤을 추출제거한다.

효소로 구성된 다량의 단백질과 함께 지질 및 콜레스테롤류등으로 결합되어 있으며 이 지질은 산화 또는 환원을 야기시키는 반응성물질로 효소단백질의 변성을 유발시킨다. 단백질의 변성 없이 지질 및 콜레스테롤류를 제거할 수 있을 경우 효소의 안정성 및 효소의 역가를 상승시킬 수 있는데, 물론 불순물인 지질등 비극성 물질은 종래의 방법인 속슬레트 soxhlet 추출장치와 노말-헥산 n-hexane , 에탄올 ethanol 또는 에텔 ether 등을 추출용매로 하여 추출을 수행할 수 있지만 추출후 용매의 완전한 회수가 어려울뿐만아니라 효소단백질의 변성을 초래하므로, 본 발명에서는 가압가스를 용매로서 사용하여 지질 및 콜레스테롤을 추출제거하고 가압가스는 회수하므로서 유기용매추출로 인한 결점을 해결할 수 있었던 것이다.

The pretreated animal pancreas is passed through a pressurized gas to extract and remove lipids and cholesterol, which are nonpolar substances. It is combined with lipids and cholesterols together with a large amount of protein composed of enzymes.

This lipid is a reactive substance that causes oxidation or reduction and causes degeneration of enzyme protein. If lipids and cholesterol can be removed without denaturation of proteins, enzyme stability and enzyme titer can be increased. Of course, nonpolar substances such as lipids are impurities such as Soxhlet extractor and normal-hexane.

Extraction may be performed using n-hexane , ethanol, or ether as an extraction solvent, but it is difficult to completely recover the solvent after extraction and also cause denaturation of the enzyme protein. By using as a solvent to extract and remove lipids and cholesterol and recover the pressurized gas was able to solve the defects due to organic solvent extraction.

본 발명에서 사용하는 가압가스로는 이산화탄소, 에틸렌, 에탄, 프로판, 아세틸렌 또는 암모니아가스등 효소에 변성을 주지 않는 범위내에 가스를 사용할 수 있으나 이중에서도 이산화탄소 가압가스의 사용이 효소의 안정성 면에서 가장 바람직하였다. As the pressurized gas used in the present invention, gas may be used within the range that does not denature the enzyme such as carbon dioxide, ethylene, ethane, propane, acetylene, or ammonia gas, but the use of pressurized carbon dioxide gas is most preferable in terms of enzyme stability.

가압가스는 주어진 온도에서 포화압력 saturation pressure 에 도달하면 기체에서 액체로 변화게 되어 이상기체 ideal gas 에서 실제기체 real gas 로 물성이 바뀌게 된다. 포화압력 이상의 압력에서 가압가스는 밀도 density 가 증가된 비극성 유체로 되어 혼합물질내의 지질, 콜레스테롤류등 비극성물질들을 용해할 수 있는 용매력 solvating power 증가와 일반적인 액체들에 비해 점도 viscosity 가 작아 빠른 이동특성과 확산능력을 수반하여 추출속도를 향상시킨다.

Pressurized gas changes from gas to liquid when the saturation pressure is reached at a given temperature, and the physical property changes from ideal gas to real gas. At pressures above the saturation pressure, the pressurized gas becomes a nonpolar fluid with increased density, increasing solvent power to dissolve nonpolar substances such as lipids and cholesterol in the mixture, and viscosity compared to other liquids.

Small improves the extraction speed with fast moving characteristics and diffusion capacity. 가압 이산화탄소로 추출이 수행되면 효소단백질을 포함한 추출잔류물질은 추출탑에 남게 되고 비극성물질인 지질과 콜레스텔류는 이산화탄소와 함께 추출탑에서 분리조로 보내지게 되어 여기서 지질과 콜레스텔류는 분리제거되고 이산화탄소는 다음 공정을 위하여 회수되어 재사용하게 된다.

When extraction is carried out with pressurized carbon dioxide, residues containing enzyme proteins remain in the extraction tower, and lipids and cholesterols, which are non-polar substances, are sent to the separation tank together with carbon dioxide from the extraction tower.

Carbon dioxide is recovered and reused for the next process. 추출잔류물질 이하 "추잔물질"이라 한다 은 가압 이산화탄소와 보조용매로 2차 추출하게 되는데, 이때 보조용매로는 물 또는 에탄올중 에탄올이 좋은데 사용량은 이산화탄소와 약 비율로 혼합하여 사용하는 것이 좋다. Extractive residues hereinafter referred to as "tuft residues" are secondaryly extracted with pressurized carbon dioxide and co-solvent, where co-solvent is preferably ethanol in water or ethanol.

Good to do. The temperature and pressure in the second extraction are the same as in the first extraction. 이산화탄소는 1차 추출시와 마찬가지로 분리조로 보내어 회수되고 사용된 보조용매는 여과하여 재사용한다. As in the first extraction, carbon dioxide is sent to a separation tank for recovery, and the co-solvent used is filtered and reused.

최종적으로 남은 단백질은 분리조에서 회수되고 단백질 함량 및 효소역가 등을 검정한 결과 고순도, 고역가의 판크레아틴임을 확인하였다. Finally, the remaining protein was recovered from the separation tank and assayed for protein content and enzyme titer to confirm that it was high purity and high titer of pancreatin.

Protein isolated after the second extraction can be separated into individual components of α-amylase, furotese, lipase contained therein as pancreatin, the type and extraction conditions of the surfactant for each enzyme in Table 1 below This can be done simply by performing a different reverse micelle method.

본 발명의 제조공정과 장치에 대하여 도면과 함께 설명하면 다음과 같다. The manufacturing process and apparatus of the present invention will be described with reference to the drawings. 도 1은 본 발명의 제조공정개략도이고, 1 is a manufacturing process schematic diagram of the present invention,. 도 2는 본 발명의 제조공정에 따른 장치도로서, 주요부분에 대한 설명을 하면, 1은가압가스탱크, 2는 냉온조, 3은 고압펌프, 4는 압력표시기, 5는 추출탑, 6은 온도감지기, 7은 압력조절기, 8은 분리조, 9는 가스미터기, 10은 열교환기, 11은 팽창코일이고, 12는 보조용매탱크이다.

Figure 2 is a device diagram according to the manufacturing process of the present invention, when explaining the main parts, 1 is a pressurized gas tank, 2 is a cold and cold tank, 3 is a high pressure pump, 4 is a pressure indicator, 5 is an extraction tower, 6 is Temperature sensor, 7 is pressure regulator, 8 is separation tank, 9 is gas meter, 10 is heat exchanger, 11 is expansion coil, 12 is co-solvent tank.

상기 장치구조는 용매저장고로부터 가압가스를 고압으로 전달시키는 압착장치, 추출온도를 일정하게 유지시키기 위한 열교환장치, 원료로부터 용매속으로 선택적인 물질이동이 일어나는 추출탑, 압력조절기를 통과하여 용매와 용질 추출물질 이 분리되는분리조, 그리고 분리조를 통과하는 가압가스를 용매로 다시 사용케하는 재순환장치등으로 이루어진다. 본 발명장치에 사용된 수송관은 부식을 방지할 수 있는 스테인레스강을 사용하였으며, 또한 밸브, 필터류는 고압용 스테인레스강을 사용한다.

추출탑은 병열로 연결되어 생산량을 조절할 수 있으며, 분리조는 파이렉스 유리 또는 스테인레스강으로 제작된다. 가압가스는 원료내의 고분자, 휘발성 비극성 물질들을 추출할 수 있지만 극성물질 등을 추출하기 위해 보조용매를 투입한다. The apparatus structure is a compression device for transferring the pressurized gas from the solvent reservoir to a high pressure, a heat exchanger for maintaining a constant extraction temperature, an extraction tower to selectively move the material from the raw material into the solvent, the solvent and the solute through the pressure regulator It consists of a separation tank in which extract material is separated, and a recirculation device for reusing the pressurized gas passing through the separation tank as a solvent.

The transport pipe used in the apparatus of the present invention used stainless steel that can prevent corrosion, and the high pressure stainless steel is used for valves and filters.

Extraction towers can be connected in parallel to control production, and the separator is made of Pyrex glass or stainless steel Pressurized gas can extract polymers and volatile nonpolar substances in the raw material, but co-solvents are added to extract polar substances.

본 발명에 따른 추출공정을 장치와 함께 개략적으로 설명하면, 추출용매인 가압가스가 탱크로부터 방출되어 냉온조 2 를 통과하는 동안 가압가스내의 기포가 제거되어 고압펌프 3 에 의해서 가압되어지며, 추출탑 5 내의 압력은 압력조절기 7 와 밸브에 의해 유지되어 압력표시기 4 에 나타내어진다.

주용매인 가압가스와 보조용매는 예열 preheat 되어 온도감지기 6 에 의해 추출탑에 유입되는 온도와 추출탑을 나가는 온도가 측정된다. 가압가스는 압력조절기에 의해 팽창되어 열교환기 10 를 통과하여 분리조 8 에서 추출물질을 침적시킨후 재사용되어 반복고정이 진행되게 되는 것이다. The pressure in the extraction column 5 is maintained by the pressure regulator 7 and the valve and is shown on the pressure indicator 4.

The pressurized gas and the co-solvent, which are the main solvents, are preheated, and the temperature flowing into the extraction tower and the temperature exiting the extraction tower are measured by the temperature sensor 6. The pressurized gas is expanded by a pressure regulator to pass through the heat exchanger 10 to deposit the extract material in the separation tank 8 and reuse it to proceed with repeated fixing.

이하 본 발명의 실시예를 구체적으로 기재한다. Hereinafter, the embodiment of the present invention will be described in detail. 판크레아틴의 제조 Preparation of Pancreatin.

전처리공정 Pretreatment process. 건강한 돼지를 도살하여 췌장을 얻은후 깨긋이 수세하여 지방과 피등을 제거한다. 동결건조된 돼지췌장을 분쇄기로 마쇄한후 35 매쉬의 체로친다. 향후, 수율관리를 위하여 수분 및 지질 등의 함량을 측정한다. Slaughter healthy pigs to obtain a pancreas, then flush with water to remove fat and blood.

Finely slice the fat-derived pancreas into a freezer at ° C and store it. The lyophilized pig pancreas is ground with a grinder and sifted through 35 mesh sieves. In the future, the content of moisture and lipids is measured for yield management.

동결건조된 돼지췌장으로부터 판크레아틴의 제조 Production of Pancreatin from Frozen Porcine Pancreas.

Enzymes are globular proteins pdocess control Digestive enzyme production process reactions. These reactions occur outside of the cells dnzyme the gut. Enzymes are Calorie counting for improved health according to the type producrion chemical reaction catalysed. All digestive enzymes are hydrolases, whereas most of the enzymes involved in energy release for muscular contraction are oxidation-reduction enzymes such as oxidases, hydrogenases and dehydrogenases. Enzymes are large protein molecules, all of which have their own specific 3D shape. The analogy that is often used to describe this mechanism is that of a key fitting into a lock. Digestive enzyme production process

Author: Faugami

2 thoughts on “Digestive enzyme production process

Leave a comment

Yours email will be published. Important fields a marked *

Design by ThemesDNA.com