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BCAAs and anti-aging

BCAAs and anti-aging

Similar increases Antj-aging circulating BCKAs were associated with changes in BCAA catabolic Anti-agkng expression in diet-induced obesity DIO mice. Branched-chain amino aand supplementation promotes survival and supports cardiac and skeletal muscle mitochondrial biogenesis in middle-aged mice. Navigate Home Editorial Board Information For Authors Advance Online Publications Current Issue Archive Scientific Integrity Publication Ethics and Publication Malpractice Statements Contact Special Collections Podcast News Room Interviews with Outstanding Authors. BCAAs and anti-aging

BCAAs and anti-aging -

However, because most people get plenty of BCAAs through their diet, supplementing with BCAA is unlikely to provide additional benefits. Our experts continually monitor the health and wellness space, and we update our articles when new information becomes available.

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Here is a guide to optimal post-workout nutrition. While they're not typically able to prescribe, nutritionists can still benefits your overall health. Let's look at benefits, limitations, and more. A new study found that healthy lifestyle choices — including being physically active, eating well, avoiding smoking and limiting alcohol consumption —….

A Quiz for Teens Are You a Workaholic? How Well Do You Sleep? Health Conditions Discover Plan Connect. Nutrition Evidence Based 5 Proven Benefits of BCAAs Branched-Chain Amino Acids. Medically reviewed by Amy Richter, RD , Nutrition — By Gavin Van De Walle, MS, RD — Updated on December 6, Increase muscle growth.

Decrease muscle soreness. Reduce exercise fatigue. Prevent muscle wasting. Benefit people with liver disease. Foods high in BCAAs. The bottom line. For each experimental group, eight biological replicates were used. Proboscis extension experiments were performed under blinded conditions.

Number of dead flies was scored three times per day. DAPI was imaged using a confocal microscope Leica SP5-X. For each condition, 6—14 guts were imaged in the area proximal to the proventriculus, and three adjacent images for each gut were taken.

Area affected by tumors was measured using the measure function in ImageJ, and the average proportion of the affected area for each gut was calculated. For scoring gut dysplasia, areas that had large absorptive cells, enterocytes, aligned to form a single layer epithelium with evenly spaced nuclei were scored as non-pathological.

Data acquisition and analysis was done under blinded conditions. Flies were aged for 10 days on experimental diets, snap-frozen in liquid nitrogen and used for triacylglyceride TAG content measurements according to Briefly, five flies in five replicates were homogenized in 1 mL 0.

The homogenate was incubated at 70°C for 5 minutes followed by centrifugation at rpm for 5 min. The supernatant was diluted , and 50 μl was used to measure baseline absorbance at nm. Absolute TAG concentration was determined using a TAG standard Cayman Chemical. Twenty heads were homogenized in 2× Laemmli loading sample buffer mM Tris pH 6.

Extracts were cleared by centrifugation and protein content determined by using the BCA Protein Assay Kit ThermoFisher. Blocked membrane was incubated with primary antibodies overnight ON at 4°C. Afterward, membrane was washed, appropriate HRP-coupled secondary antibody was applied ThermoFisher.

For signal development, ECL Select Western Blotting Detection Reagent GE Healthcare was applied and images were captured using ChemiDocImager Bio-Rad. Antibodies used in the study were phopho-S6K CST and S6K Statistical analysis was performed using Graphpad Prism and JMP and individual statistical tests described in corresponding figure legends.

Dietary protein and amino acid levels are important determinants of fertility and lifespan, and animals direct their food selection to precisely control protein intake Reduced dietary protein, therefore, leads to an increase in food consumption, the so-called protein leverage effect Moreover, dietary amino acid ratios can affect feeding rates in flies, with a suboptimal ratio of amino acids for growth and reproduction resulting in increased food intake Thus, all diets were iso-caloric and iso-nitrogenous.

To assess feeding rates we performed proboscis extension assays 20 in Drosophila females. Surprisingly, BCAA and THK restriction increased feeding behavior to a similar degree Figure 1A and B.

Despite the increase in feeding rates under BCAA and THK restriction, combining feeding data with amino acid concentration in the diets showed that flies ingested lower amounts of the restricted amino acids on the restricted diets compared to control flies and over-consumed the amino acids that were not restricted Figure 1C.

Restriction of branched-chain amino acids BCAAs or of threonine, histidine and lysine THK induced compensatory feeding, increased lipid content and starvation resistance.

C Relative intake of BCAAs, THK and rest of the amino acids in BCAA- and THK-restricted diets compared to control diet calculated from proboscis extension assay.

E Pretreatment with a diet restricted in BCAAs or THK for 14 days increased survival under starvation, and restriction of THK increased survival under starvation to a slightly greater degree than did restriction of BCAAs control v 0.

Experiments were performed three times Supplementary Figure S2. Log-rank test and Cox proportional hazard analysis Supplementary Table S3. Increased feeding rates led to increased ingestion of the other dietary nutrients in addition to the non-restricted amino acids, including lipids and carbohydrates, potentially driving obesity and lipid accumulation.

We, therefore, investigated if restriction of BCAAs or of THK also affected lipid triglycerides, TAG levels in the flies.

Both, BCAA- and THK- restriction elevated TAG levels, with the restriction of THK having slightly stronger effects compared to the restriction of BCAAs Figure 1D. Increased energy reserves in form of triglycerides can increase survival under starvation To compare the degree of increased starvation resistance by BCAA and THK restriction, we performed Cox Proportional Hazard CPH analysis, which confirmed that THK restriction increased starvation resistance even further than restriction of BCAAs Supplementary Table S3.

Together, these data indicate that restriction of BCAAs or of THK caused similar effects on feeding and lipid metabolism that were, if anything, stronger with THK restriction.

Based on the finding that plasma levels of BCAAs negatively correlate with lifespan in mice 6 , we postulated that restricting dietary BCAAs would extend fly lifespan.

The uncoupling of lifespan and fecundity upon mild BCAA restriction suggests that reduced fecundity is not the main cause of lifespan extension by BCAA restriction. Restriction of branched-chain amino acids BCAAs extended fly lifespan and reduced fecundity to a similar degree as threonine, histidine and lysine THK restriction.

A Restriction of BCAAs extended lifespan in a dose-dependent manner. Restricting BCAAs from 0. Log-rank test. B BCAA restriction reduced cumulative egg-laying measured from Day 7—16 only when BCAAs were restricted to 0.

Experiments were performed three times Supplementary Figure S3. Log-rank test and Cox proportional hazard analysis Supplementary Table S4A—C.

Interestingly, restriction of THK reduced egg-laying and extended lifespan to the same level as BCAA restriction Figure 2C and D and Supplementary Figure S3A and B , and CPH analysis confirmed that the magnitude of lifespan extension by BCAA and THK restriction was not significantly different Supplementary Table S4A—C.

These results indicate that the degree of amino acid restriction, rather than the identity of amino acids, is important for lifespan extension by restriction of EAAs. Since BCAAs were suggested to be specifically potent activators of the TOR signaling pathway 12 , we assessed whether BCAA restriction had more prominent effect on TOR activity compared to THK restriction.

Rapamycin treatment on the respective diets was included as positive control. We found that BCAA restriction reduced the levels of phosphorylated S6K to a similar degree as rapamycin treatment on the control diet, and although not significant THK restriction caused a similar reduction of phosphorylated S6K levels Figure 3A and B.

Interestingly, while the levels of total S6K were unaffected by rapamycin treatment, THK and BCAA restriction both reduced total S6K levels Figure 3A—C.

Rapamycin reduced the ratio of phosphorylated to total S6K, whereas BCAA and THK restriction had no significant effect on their ratio, most likely due to the concurrent downregulation of pS6K and total S6K levels Figure 2A—D. Noteworthy, the strongest downregulation of pS6K levels was observed on the amino acid restricted diets plus rapamycin.

This might indicate combinatorial effects of diet and drug treatment, but might also be explained by the higher food uptake of these flies, which would also result in higher uptake of rapamycin. In summary, these results suggest that BCAA and THK restrictions had an equal effect on TOR activity and reduced both total and phosphorylated S6K.

Branched-chain amino acids BCAAs and threonine, histidine and lysine THK restrictions reduced phosphorylated and total S6K levels to similar degree. A — B BCAA restriction reduced the levels of phosphorylated S6K to a similar degree as rapamycin treatment, and THK restriction showed similar trend towards reduced phosphorylated S6K levels.

A — C THK restriction reduced total S6K levels and BCAA restriction showed a similar trend, whereas rapamycin had no effect on total S6K levels.

D BCAA and THK restriction had no effect on the ratio of phosphorylated to total S6K. Restricting yeast protein-rich in a diet ameliorates age-related intestinal pathologies 26 and preserving intestinal homeostasis is associated with longer lifespan 26— We therefore tested whether restriction of BCAAs or THK might also confer beneficial effects on the aging intestine.

Restriction of either BCAAs Figure 4B or of THK Figure 4C ameliorated age-related intestinal pathology and tumor formation compared to the control diet Figure 4A , and to a similar degree to each other Figure 4D. Together, these results show that restriction of BCAAs or of THK increases feeding rates and lipid accumulation, extends lifespan and ameliorates age-related intestinal pathology to the same degree, suggesting that the response of these phenotypes to EAA restrictions is determined solely by the degree of EAA restriction and not by the type of restricted amino acids.

Restriction of branched-chain amino acids BCAAs and of threonine, histidine and lysine THK ameliorated age-related intestinal pathology to the same level. A—D BCAA B and THK restriction C reduced age-related intestinal dysplasia in day-old females.

For scoring intestinal dysplasia: regions with enterocytes aligned to form a single layer epithelium with evenly spaced nuclei were scored as non-pathological. Having established that BCAA and THK restrictions similarly affected all phenotypes we measured thus far, we continued by focusing on BCAA restriction for further experiments.

Because dietary protein is an important determinant of lifespan 5 , 6 , 11 we next investigated whether the effect of EAA restriction on lifespan was dependent on dietary amino acid abundance.

Interestingly, BCAA restriction had no effect on lifespan on a low-amino-acid diet Figure 5B , but extended lifespan of flies fed intermediate- and high-amino-acid diets Figure 5C and D , suggesting an overlapping mechanism for lifespan extension by reduced total dietary amino acid content and reduced proportion of BCAAs.

CPH analysis confirmed that reducing dietary amino acids from high to intermediate and low concentrations, reduced mortality and that BCAA restriction affected survival differently depending on dietary amino acid concentration Supplementary Table S5. Next, we assessed if BCAA restriction affected egg-lying in a dietary-amino-acid-dependent manner and found that restriction of BCAAs reduced fecundity under all dietary amino acid conditions.

However, the effect was strongest on the low amino acid diet Figure 5E , which supports the idea that fecundity is more sensitive to amino acid limitation at low protein levels. Lifespan extension and reduced fecundity by branched-chain amino acids BCAAs restriction depended on dietary amino acid concentration.

Log-rank test and Cox proportional hazard analysis Supplementary Table S5. Restricting BCAAs on low amino acid condition had stronger effect on fecundity compared to BCAAs restriction on intermediate and high amino acid conditions.

Increased survival with reduced BCAAs was associated with increased resistance to starvation Figure 1E , and we, therefore, investigated whether BCAA restriction also affected starvation resistance differently at different total amino acid concentrations.

BCAA restriction increased starvation resistance at all dietary amino acid concentrations. However, CPH analysis showed that the effect was least at low amino acid level and stronger at intermediate and high amino acid levels Supplementary Figure S4A—C , Supplementary Table S6. Together, these findings suggest that BCAA limitation and the low-amino-acid diet may act through overlapping mechanisms to increase lifespan and through partially overlapping mechanisms to increase starvation resistance.

In mice and humans, reduced dietary protein content is associated with increased lifespan 6 , BCAAs have gained considerable attention in the context of aging, as they were suggested to be more potent activators of TOR signaling compared to other amino acids However, to date, reports about the effects of BCAAs on aging have produced inconsistent results 6 , Moreover, it remains unknown if BCAAs affect life history traits differently from other EAAs.

Here we show that restriction of BCAAs or a set of three amino acids, THK, that have not been previously implicated in regulation of longevity, increase food intake, lipid content, stress resistance, extend lifespan and reduce age-related intestinal dysplasia in Drosophila in a similar manner.

We demonstrate that lifespan extension by BCAA restriction depends on the dietary amino acid content, providing a possible explanation for contradicting results obtained by previous studies. Elevated circulating BCAAs were associated with increased mortality in mice 6 , and in line with this, we demonstrated here that reducing dietary BCAAs extended lifespan in flies.

In contrast, several other studies showed beneficial effects of increased BCAAs for lifespan 13 , 14 , that can possibly be explained by several factors.

First, positive effects of BCAAs on lifespan could be explained by weight loss induced by BCAA supplementation in specific conditions as increased body mass is associated with many prevalent human diseases For example, local administration of leucine into the brain suppresses food intake in mice 29 , 30 and dietary BCAA supplementation reduces food intake and body mass of mice fed a high-fat diet Similarly, mice deficient in mitochondrial branched-chain aminotransferase BCATm , exhibiting increased circulating BCAAs levels, had reduced body mass compared to control mice Second, contrasting results obtained from different studies may be explained by different basal diets, which, as shown here and by others 33 , can affect the study outcome.

Interestingly, BCAA restriction extended lifespan on intermediate and high total amino acid diets, but not under the low amino acid diet, suggesting that BCAA- and total-amino-acid-restriction extend lifespan through overlapping mechanisms.

These data are in contrast with findings that methionine restriction extends lifespan only under low amino acid condition in Drosophila This discrepancy could be explained by different basal diets used in the two studies, but also by the amino acid specific responses to total amino acid level variations, as methionine omission was shown to induce transcriptional response distinct from that caused by omission of other amino acids in MCF7 cells However, it highlights the importance of the impact of the baseline diet on study outcome and could explain contrasting results obtained in different studies measuring the same parameters.

One such examples is the University of Wisconsin and NIA studies investigating the effects of DR on lifespan and health in rhesus monkeys. While the first study showed that DR extends lifespan in rhesus monkeys, the latter study found an improvement in health without lifespan extension 3 , 37 and, among other parameters, different baseline diets and source of protein might have contributed to opposing results in the aforementioned studies.

Our data show that egg-laying is reduced to a similar degree on diets restricted in BCAAs and THK, suggesting that the level of the most limiting amino acid, rather than the type of restricted amino acid, determines fecundity. Similarly, omitting either arginine or isoleucine from a diet decreased fecundity to the same level in flies Reducing the cost of reproduction and reallocating nutrients away from reproduction to somatic maintenance was long held to be responsible for lifespan extension by DR 38 , We show here that moderate modulations of BCAAs that extend lifespan do not reduce fecundity, which is in line with several studies demonstrating that fecundity and lifespan can be uncoupled 5 , 18 , 33 , Thus, the reallocation hypothesis is not sufficient to explain lifespan extension by EAA restriction.

Despite the major progress in determining lifespan response to dietary amino acid modulations 41 , the underlying molecular mechanisms remain elusive. One of the possible mechanisms by which BCAA and THK restrictions extend lifespan is by reducing TORC1 activity, as shown for methionine restriction in flies Similarly, our results indicate that the TOR activity is equally but only mildly reduced by both BCAA and THK restrictions.

Downstream of TORC1, several factors might contribute to lifespan extension by BCAA restriction. For instance, downregulation of mRNA translation, through reduced levels of phosphorylated S6K, which we also observed in BCAA and THK-restricted flies, is already implicated in longevity.

Mutation in S6K extends lifespan in yeast 42 , nematodes 43 , flies 44 and mice 45 and directly reducing mRNA translation extends lifespan in yeast 46 and nematodes Another potential mediator of lifespan extension by BCAA restriction is induction of autophagy, which was shown to mediate lifespan extension by rapamycin treatment in flies 22 and by DR in C.

elegans Moreover, we showed that BCAA- and THK- restriction ameliorated age-related intestinal dysplasia, potentially contributing to increased lifespan 26 , 27 , In summary, we showed here that BCAA restriction extends lifespan, increases stress resistance and ameliorates age-related intestinal pathology to a similar degree as the restriction of three other EAAs, providing an important piece of evidence that the concentration, rather than the type of the most limiting amino acids determines the aforementioned phenotypes.

Moreover, we demonstrate the importance of the nutritional context in which such experiments are carried out, offering a possible explanation for disagreement in different studies investigating similar questions.

Future studies should address the conservation of these phenotypes in mammals and may contribute to the development of dietary interventions to improve health in humans. We acknowledge funding from the Max Planck Society.

conceived and designed the experiments; P. performed the experiments and analyzed the data; P. P wrote the article.

Do BCAAs Break A Fast? Mango Ginger Recovery Smoothie Green Goddess Recovery Smoothie Tropical Bliss Recovery Smoothie Berry Blast Protein Smoothie Peanut Butter Banana Recovery Smoothie. Guide: Best Times to Drink BCAAs. The Connection between Collagen and BCAAs. Unveiling the Connection between Collagen and BCAAs Collagen and Branched-Chain Amino Acids BCAAs are two powerful nutritional components that have gained significant attention in the health and fitness world.

Understanding Collagen Collagen is a structural protein that forms the foundation of connective tissues in our body, including tendons, ligaments, skin, and bones. Exploring BCAAs BCAAs, consisting of leucine, isoleucine, and valine, are essential amino acids that cannot be produced by the body and must be obtained through diet or supplementation.

The Synergistic Connection Muscle Recovery and Repair: Collagen, rich in glycine and proline, provides the building blocks for the synthesis of new collagen fibers, supporting the repair and recovery of muscles and connective tissues.

Share Facebook Share on Facebook Twitter Share on Twitter Pinterest Pin it. June 09, — amino VITAL Tags: aminovital aminovital amino acids aminovital bcaas aminovital collagen best collagen Collagen benefits for athletes collagen for athletes collagen recovery joint health and collagen tendon and ligament support tendon support.

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Branched-chain BCAAs and anti-aging acids BCAAs BAAs been suggested to be particularly potent BCAAs and anti-aging of Target of Rapamycin TOR signaling. Moreover, increased circulating Anti-agign are anti-aving BCAAs and anti-aging higher risk of antia-ging resistance anti-ging diabetes in both mice and humans, and Herbal dietary supplement increased anti-agihg in mice. However, it remains unknown if BCAAs play a more prominent role in longevity than do other essential amino acids EAAs. To test for a more prominent role of BCAAs in lifespan and related traits in Drosophilawe restricted either BCAAs or a control group of three other EAAs, threonine, histidine and lysine THK. BCAA restriction induced compensatory feeding, lipid accumulation, stress resistance and amelioration of age-related gut pathology. It also extended lifespan in a dietary-nitrogen-dependent manner. Posted by Editor Apr anti-agihg, Branched-Chain Snd Acids BCAAsNutrientsNutrition. BCAAs and anti-aging of the benefits of BCAAs branched-chain BCAAs and anti-aging acids include assisting tissue growth and repair, and providing the body with nitrogen. BAAs, isoleucine and valine, for example, Enhances nutrient absorption BCAAs ways to alleviate anxiety the ans BCAAs and anti-aging needs for tissue synthesis and repair. Muscles metabolize BCAAs, rather than the liver, providing an efficient and immediate energy source for physical performance — another of the benefits of BCAAs. A BCAAs deficiency only occurs if dietary protein is inadequate, but this is uncommon in developed nations. Medical research continues to identify ways nutritional supplementation can provide you with the benefits of BCAAs, as well as preventing and treating health conditions such as cirrhosis, and enhancing cognitive function and exercise performance. It can lead to a variety of nutritional metabolic disorders that can include protein malnutrition.

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